Steroid isolation , depending on context, is the isolation of chemical matter required for chemical structure elucidation, derivitzation or degradation chemistry, biological testing, and other research needs (generally milligrams to grams, but often more  or the isolation of "analytical quantities" of the substance of interest (where the focus is on identifying and quantifying the substance (for example, in biological tissue or fluid). The amount isolated depends on the analytical method, but is generally less than one microgram.  [ page needed ] The methods of isolation to achieve the two scales of product are distinct, but include extraction , precipitation, adsorption , chromatography , and crystallization . In both cases, the isolated substance is purified to chemical homogeneity; combined separation and analytical methods, such as LC-MS , are chosen to be "orthogonal"—achieving their separations based on distinct modes of interaction between substance and isolating matrix—to detect a single species in the pure sample. Structure determination refers to the methods to determine the chemical structure of an isolated pure steroid, using an evolving array of chemical and physical methods which have included NMR and small-molecule crystallography .  : 10–19 Methods of analysis overlap both of the above areas, emphasizing analytical methods to determining if a steroid is present in a mixture and determining its quantity. 
Saponin glycosides can cause hemolysis of red blood cells ( Select Triterpenoid and Saponin-Rich Herbs ). This primarily occurs when most saponins are injected intravenously or when they are hyperabsorbed from an abnormal gut. Some saponins have been successfully injected. Normally, saponins are not well absorbed orally, and the slow rate at which they are absorbed is more than sufficient to allow the body to adapt to them. This hemolytic effect does not appear to be related to the detergent properties of saponins but instead seems to be due to increasing cell membrane permeability. Overall, oral saponins appear to be extremely safe.
As we mentioned, fenugreek contains 4-hydroxyisoleucine, the amino acid responsible for encouraging the production of insulin and lowering the rate of glucose assimilation in the intestines. This can be of particular benefit to those with diabetes for stabilizing blood sugar levels.
In one study , using the amino acid extracted from fenugreek seeds, it was concluded that "4-hydroxyisoleucine insulinotropic activity might, at least in part, account for fenugreek seeds' anti-diabetic properties."
In addition, the presence of galactomannan in fenugreek furthermore slows down the rate of blood sugar absorption. The sprouts or seeds can therefore be added to meals as a potentially effective way to prevent blood sugar spikes from high glycemic foods.
The diosgenin compound has been shown to help reduce the level of serum cholesterol. In addition, other saponins and extra components like polysaccharides, pectin, hemicellulose and mucilage are found to help lower the "bad" cholesterol (LDL) by binding to and eliminating toxins while inhibiting bile salts from being absorbed in the colon.